p-t-Butyl-calixarene bonded silica stationary phase(CBS) for high performance liquid chromatography was prepared via condensation reaction on the surface of solid .The stationary phase was characterized by means of elemental analysis and FTIR. The chromatographic performance of CBS was studied by using disubstituted benzenes as the solutes and methanol-water or methanol-buffer as the mobile phases, and compared with that obtained on CPS and ODS. The results show that the chromrtographic process can proceed in various retention mechanism, such as π-π, hydrogen-bonding, charge-transfer and inclusion complexation, besides hydrophobic interaction.
A calix[8]arene-bonded gel silica stationary phase(DCABS) was prepared by using coupling reagent(KH560). Its structure was characterized by FTIR, elemental analysis and thermal analysis. The chromatographic performance of DCABS was studied by using different solutes as probes and stationary phases as reference. The results show that the new material has an excellent reversed-phase chromatographic property. The new bonded phase can provide various sites for analytes, such as the hydrophobic interaction, hydrogen-bonding, π-π, electrostatic interactions. Meanwhile, it was observed that the chromatographic performance of DCABS is partially different from CABS because of elimination of the butyl groups.
