A calix[8]arene-bonded gel silica stationary phase(DCABS) was prepared by using coupling reagent(KH560). Its structure was characterized by FTIR, elemental analysis and thermal analysis. The chromatographic performance of DCABS was studied by using different solutes as probes and stationary phases as reference. The results show that the new material has an excellent reversed-phase chromatographic property. The new bonded phase can provide various sites for analytes, such as the hydrophobic interaction, hydrogen-bonding, π-π, electrostatic interactions. Meanwhile, it was observed that the chromatographic performance of DCABS is partially different from CABS because of elimination of the butyl groups.
Various zirconia stationary phases were prepared by modification with di-dentate Lewis bases: dodecyl-N,N-diacetic acid（RNCC）, dodecyl-N-acetic acid-N-methyleneposphonic acid（RNCP）, dodecyl-N,N-dimethylenephosphonic acid（RNPP） and dodecencylsuccinic acid（RCC）. The color reaction of RNPP-ZrO2 and RNCP-ZrO2 with ammonium molybdate indicate there are free phosphonic groups on the surface of the stationary phases, and the FTIR spectra of RNCC-ZrO2 and RNCC-ZrO2 indicate there are free carboxyl groups. The RCC-ZrO2 is far more stable than RNCC-ZrO2 under alkali station. The results of color reaction, FTIR spectra and the stability test of stationary phases suggest that, due to the chelating effect, RNCC, RNCP and RNPP adsorb on zirconia with only one carboxyl or phosphonic group, however, RCC adsorb on zirconia with two carboxyl groups.
