[ Objective] Study on the photosynthesis and influencing factors in super high-yield combination C Liangyou H255. [ Method] The photosynthetic characteristies were measured at a hot and muggy day under natural conditions. [ Result] A respective single peak at 11:30 was observed in diurnal variation curves of net photosynthetic rate (Pn) and transpiration rate(Tr). Correlation analysis shows that Pn presents an extremely significant correlation with photon flux densities (PFD) of photosynthetically active radiation, in comparison assumes a significant correlation either with stomatal conductance (Gs) or with ambient CO2 concentration (Ca). [ Conclusion] Gs followed by PFD presented most influence on Pn of super high-yield combination C Liangyou H255.