[Objective] This study aimed to investigate the effect of soil organic carbon mineralization at different temperature on the amount of nitrogen application, in order to provide references for the establishment of carbon circulation model for orchard eco-system. [Method] The effects of nitrogen treatments on soil organic carbon mineralization of citrus orchard soil were investigated under 10, 20, 30 ℃ by laboratory simulated experiment. [Result] The mineralization rate decreased quickly at the be- ginning of the experiment but remained stable at the late period under three temper- ature treatments. The amounts of CO2 ranged from 1 328.25-2 219.42 mg/kg under three temperature condition, and the amount of soil organic carbon mineralization of 100 mg/kg (N4) treatment was the greatest, while that of CK was the lowest. High level nitrogen treatment (N4 and N3) were significant higher than the lower level nitro- gen treatment (N2 and N1). The soil organic carbon mineralization rate increased with the temperature from 10 to 30℃. The dependence of soil carbon mineralization to temperature (Q10) was different under different nitrogen treatments that the Qlo value of N2 treatment was the lowest while that of the N4 treatment was the greatest. The soil organic carbon mineralization in Citrus orange orchard soil was affected significantly by high level nitrogen treatment, but with no significance under lower nitrogen treatment. [Conclusion] The dependence of soil carbon mineralization to temperature (Q10) increased with the increasing nitrogen input. The combination of nitrogen with temperature may increase the CO2 emission from Citrus orchard soil.