对从牛蒡中提取菊糖的工艺条件进行了研究.在提取过程中,通过单因素法分析了几个主要因素———料液质量比、微波提取功率和提取时间、水提取温度和提取时间对提取率的影响.在单因素的基础上,通过均匀设计法对微波辅助提取牛蒡菊糖的工艺条件进行了优化,得到的最佳提取工艺条件为:料液质量比1∶35、微波提取280 s、95℃热水提取15 m in,提取率可达93.6%.
A water soluble polysaccharide (HPⅠ) was isolated from the Hericium caput-medusae and examined homogenously by Sephadex G-100 column chromatography,cellulose acetate film electrophoresis and specific optical rotation measurement. This HPⅠconsists of Fuc,Glc and Gal in the molar ratios of 0.423∶1∶2.110. Its structural features were investigated by methylation and GC-MS analysis,periodate oxidation-Smith degradation,and partial acid-hydrolysis. In addition, enzymatic degradation experiments and GC analysis were applied. The following structure of the highly branched repeating unit was established: 6→)-Gal-(1→6)-Gal-(1→6)-Gal-(1→6)-Gal-(1→6)-Gal-(1→6)-Gal-(1→6)-Gal-(1→6)-Gal-(1→6)-Gal-(1→ 2 ↑ 1 2 ↑ 1 2 ↑ 1 Gal-(1→4)-Fuc Glc-(1→3)-Glc 2 Gal-(1→6)-Glc 3 The activities of HPⅠwere tested in mice. All the samples exhibited high effect of prevention and cure against chronic gastricism.
