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西瓜蔓枯病分子诊断技术研究被引量:9
2006年
本文测定西瓜上的西瓜蔓枯病菌(D idym ella bryoniae)、西瓜炭疽病菌(Colletotrichum orbiculare)及西瓜枯萎病菌(Fu-sarium oxysporumf.sp.niveum)的rDNA的ITS序列,比对近缘种及西瓜上不同病菌的ITS序列同源性,设计出特异性上游引物XM-2和下游引物XM-R2。经过对XM-2/XM-R2引物的PCR扩增条件的优化,可以扩增出一条344bp的西瓜蔓枯病菌特异性DNA条带。上述方法可以检测到pg以上的蔓枯病菌基因组DNA,并且可以准确扩增出西瓜蔓枯病自然病样中特异性的DNA片段。本文建立了一项西瓜蔓枯病分子检测技术,该方法准确、快速、可靠,可用于西瓜蔓枯病田间的快速诊断。
戴富明刘少华任小杰陆金萍倪秀红徐敬友
关键词:西瓜蔓枯病特异性引物
上海地区草莓炭疽病病原鉴定被引量:50
2008年
Seventeen isolates of strawberry anthracnose were obtained from Shanghai suburb.According to the temperature test for mycelial growth,these isolates were divided into two groups: one was strain CMf-04 with optimal temperature of 24℃,the other was including 16 strains with optimal temperature of 28℃,and most of which could produce sexual stage on PSA media,e.g.QPg-961.Conidia of CMf-04 were hyaline,unicellular,straight and fusiform,(12.1-16.4)μm×(3.6-5.4)μm.Conidia of QPg-961 were hyaline,unicellular and ovoid to oblong,(13.0-19.7) μm×(4.1-7.3)μm.On basis of morphologcal,biological characteristics and the sequences of ribosome rDNA ITS,isolate CMf-04 from the strawberry rotten fruits was identified as Colletotrichum acutatum;while all the other isolates from the diseased leaf stalks,runners and root crowns at strawberry plantlet propagation stage were belonged to Colletotrichum gloeosporioides.It proved that C.gloeosporioides was the main pathogen of the strawberry anthracnose in summer strawberry propagation fields in Shanghai and it is of significance for the breeding of resistant strawberry and its control.
任小杰梁艳陆金萍杨百荣徐敬友戴富明
关键词:草莓病害病原鉴定炭疽病营养丰富
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