Seventeen isolates of strawberry anthracnose were obtained from Shanghai suburb.According to the temperature test for mycelial growth,these isolates were divided into two groups: one was strain CMf-04 with optimal temperature of 24℃,the other was including 16 strains with optimal temperature of 28℃,and most of which could produce sexual stage on PSA media,e.g.QPg-961.Conidia of CMf-04 were hyaline,unicellular,straight and fusiform,(12.1-16.4)μm×(3.6-5.4)μm.Conidia of QPg-961 were hyaline,unicellular and ovoid to oblong,(13.0-19.7) μm×(4.1-7.3)μm.On basis of morphologcal,biological characteristics and the sequences of ribosome rDNA ITS,isolate CMf-04 from the strawberry rotten fruits was identified as Colletotrichum acutatum;while all the other isolates from the diseased leaf stalks,runners and root crowns at strawberry plantlet propagation stage were belonged to Colletotrichum gloeosporioides.It proved that C.gloeosporioides was the main pathogen of the strawberry anthracnose in summer strawberry propagation fields in Shanghai and it is of significance for the breeding of resistant strawberry and its control.