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张媛

作品数:6 被引量:35H指数:3
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发文基金:国家自然科学基金深圳市科技计划项目国家重点基础研究发展计划更多>>
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艾灸干预胃溃疡大鼠胃黏膜脑肠肽的动态表达被引量:11
2019年
目的:观察艾灸干预胃溃疡大鼠胃黏膜相关脑肠肽的动态表达,探讨艾灸干预胃溃疡的时效关系。方法:SD大鼠72只随机分为3批,各批分为正常组、模型组、胃经穴位组和非经穴位组(n=6)。采用70%乙醇灌胃复制胃溃疡大鼠模型。胃(非)经穴位组的大鼠进行艾灸1、4、7d(30min/d)。干预结束后于光镜下观察胃黏膜形态,RT-PCR测定胃黏膜增殖细胞核抗原(PCNA)及P物质(SP)表达。结果:与正常组比较,模型组大鼠胃黏膜溃疡灶明显,胃黏膜PCNA表达降低(P<0.05),SP表达升高(P<0.05);与模型组比较,干预1d后胃经穴位组大鼠胃黏膜PCNA表达升高(P<0.05),干预4d后胃经穴位组PCNA表达升高(P<0.05),SP降低(P<0.05);干预7d后各组大鼠胃黏膜PCNA及SP表达无显著差异。结论:艾灸胃经穴位能有效调节相关脑肠肽指标,促进胃黏膜损伤修复,并具有一定的时效关系。
何其达刘密连林宇张媛沈佳成刘彩春黄妙森张珑滨钱林超钱林超常小荣
关键词:艾灸胃溃疡胃经时效关系脑肠肽
Transverse striation needling for 30 cases of cervical spondylosis被引量:2
2017年
Objective To observe the clinical efficacy of transverse striation needling in treatment of cervical spondylosis.Method Thirty patients were diagnosed with cervical spondylosis in Guoyitang Clinic,Yanwu Branch of Zhongshan Hospital,Xiamen University from July,2013 to March,2015,and were included into this study.Transverse striation needling,i.e.transverse insertion into the transverse striation of the cervical vertebra,was adopted for the 30 patients,and conventional acupuncture at Fēngchí(风池 GB 20,bilateral),Jiānj?ng(肩井 GB 21,bilateral) and Jiānzhōngshù(肩中俞 SI 15,bilateral) was combined.The treatment was conducted for once a day,twice a week,and 6 times were needed in total.Follow-up visit was performed two months after the treatment.Result Among the 30 patients,the markedly effective rate was 70.0%(21/30),effective rate was 30.0%(9/30),and the total effective rate was 100.0%.There was no relapse during two months after the treatment.Conclusion The therapeutic effect of transverse striation needling in treatment of cervical spondylosis was good.
张媛马富强杨宗保刘彩春
关键词:ACUPUNCTURE
电针“梁门”-“足三里”对急性胃黏膜损伤模型大鼠血清代谢物动态表达的影响被引量:4
2019年
目的采用核磁共振氢谱技术(1H NMR)探讨电针梁门-足三里治疗急性胃黏膜损伤的可能作用机制。方法 54只SD大鼠随机分为正常组、模型组、电针组,各组又分为干预1、4、7天3个时间点,每个时间点6只。除正常组外其余各组采用75%乙醇灌胃法复制急性胃黏膜损伤大鼠模型。造模成功后电针组大鼠用鼠板捆绑固定,采用疏密波(4 Hz,50 Hz),电针"梁门"(负极)"足三里"(正极),每次30 min,每日1次。正常组和模型组大鼠用鼠板捆绑固定30 min,每日1次。分别于干预1、4、7天后光镜观察胃黏膜病理形态,检测大鼠血清1H NMR谱,并利用模式识别方法分析不同干预时间下代谢物谱的差异,用正交偏最小二乘判别分析(OPLS-DA)模型和独立样本t检验,以P <0. 05筛选潜在的生物代谢标志物。结果病理结果显示,正常组大鼠胃黏膜表面细胞完整,未见溃疡性损害。干预1、4天时模型组大鼠可见胃黏膜上皮结构破坏严重,毛细血管增生及出血;干预4天时电针组大鼠胃黏膜的黏膜结构恢复较为完整,较模型组明显改善。血清代谢组学结果显示,正常组与模型组分离出差异代谢物6个:甘油磷酸胆碱、谷氨酰胺、甜菜碱、葡萄糖、乙酸盐和胆碱。与模型组比较,干预1天时电针组胆碱、甘油磷酸胆碱、葡萄糖、乙酸盐、谷氨酰胺和甜菜碱均升高;干预4天时胆碱、葡萄糖、谷氨酰胺和甜菜碱升高,甘油磷酸胆碱和乙酸盐降低;干预7天时甘油磷酸胆碱降低,葡萄糖、乙酸盐、谷氨酰胺和甜菜碱升高,胆碱无明显变化。结论电针"梁门"-"足三里"可有效回调急性胃黏膜损伤大鼠血清相关差异代谢物,恢复相应代谢通路稳定,以电针4天效果最佳,可能是其逆转胃黏膜损伤的作用机制之一。
连林宇刘彩春钱林超刘密何其达张媛张媛黄妙森张珑宾杨宗保
关键词:急性胃黏膜损伤电针梁门足三里核磁共振
Excitement transfer therapy for 32 cases of insomnia
2017年
Objective To observe the clinical efficacy of excitement transfer therapy for insomnia. Methods According to the inclusion criteria, 32 patients, selected from Guoyitang, affiliated Zhongshan Hospital Yanwu Branch of Xiamen University from May to October, 2016, were diagnosed with insomnia and included in this study. All the patients were treated by adopting the excitement transfer therapy, i.e. cluster needling was carried out at the acupoints of wrist and ankle in the manner of penetration needling, and combined with sliding- cupping at governor vessel and bladder meridian on the back. All the subjects were treated for 3 times per week, and treatment for 6 times was considered as I course of treatment. The curative effect was observed after treatment for 2 courses, and follow-up visit was performed for two months after treatment. Efficacy evaluation criteria were established by reference to Guidelines of Clinical Research on Chinese New Herbal Medicine (Trial) Results Cured: 11 cases (34%4 markedly effective: 16 cases (50%), effective: 4 cases (13%4 ineffective: 1 case. The total effective rate was up to 97%. There was no relapse during two months after the treatment. Conclusion The efficacy of excitement transfer therapy for insomnia was significant, which was worthy of clinical promotion and application.
陈娇龙刘彩春何其达连林宇马富强王红沈佳成张珑宾张媛杨宗保
关键词:INSOMNIAEFFICACY
电针对胃黏膜损伤大鼠胃黏膜组织饥饿素与生存素动态表达的影响被引量:2
2020年
目的:观察电针治疗急性胃黏膜损伤大鼠过程中不同节点的饥饿素(Ghrelin)和生存素(Survivin)差异表达,探讨电针治疗急性胃黏膜损伤的时效关系。方法:将72只SD大鼠根据干预时间随机分为1、4、7d3批,各批又分成4个亚组:正常组、模型组、胃经穴位组和胆经穴位组,每组6只。采用75%乙醇灌胃法复制急性胃黏膜损伤大鼠模型,并分别对胃(胆)经穴位组的大鼠进行电针干预1、4、7d(30min/d)。干预结束后于光镜下观察胃黏膜形态,Western Blot测定胃黏膜脑肠肽Survivin及Ghrelin的动态表达,并分析和比较其随干预治疗的进程出现的变化。结果:干预1d,模型组大鼠胃黏膜溃疡灶明显,Ghrelin降低(P<0.01);与模型组比较,胃经穴组的Ghrelin和Survivin皆升高(P<0.01,P<0.05),胆经穴组的Ghrelin升高(P<0.05)。干预4d,模型组溃疡程度有所好转,Survivin降低(P<0.05);与模型组比较,胃经穴和胆经穴组的Ghrelin与Survivin均明显升高(P<0.01,P<0.05)。干预7d,模型组溃疡程度已接近正常,其中Ghrelin升高(P<0.05),Survivin降低(P<0.05);与模型组比较,胃经穴组Ghrelin与Survivin均显著升高(P<0.05),胆经穴组的Survivin升高(P<0.05)。结论:电针胃经穴能够不断的激活Survivin与Ghrelin的大量表达,从而达到促进胃溃疡修复的最终目的。
连林宇沈佳成何其达张媛黄妙森张珑宾常小荣杨宗保
关键词:急性胃黏膜损伤脑肠肽生存素
电针促进胃黏膜损伤修复的时效关系及分子机制被引量:17
2018年
目的:动态观察电针对胃溃疡模型大鼠胃黏膜损伤修复的影响,探讨电针治疗胃溃疡的时效关系和分子机制。方法:72只SD大鼠分为空白组、模型组、胃经穴组、对照点组,并按干预时间1、4、7 d分为3个亚组,每个亚组6只。采用乙醇灌胃方法制备胃溃疡大鼠模型,胃经穴组电针"足三里""梁门",对照点组电针"梁门""足三里"外旁开5 mm处,每日1次,每次电针30 min。空白组和模型组用鼠板束缚但不进行电针处理,每日1次,每次30 min。用逆转录-聚合酶链反应(PR-PCR)法检测胃增殖细胞核抗原(PCNA)、P物质(SP)的表达,Western blot检测胃神经降压素(NT)的表达。结果:干预1 d后,模型组溃疡指数显著高于空白组(P<0.01),PCNA、SP、NT表达降低(P<0.01,P<0.05);胃经穴组与模型组、对照点组相比,溃疡指数降低(均P<0.05),上调PCNA m RNA、SP m RNA的表达(均P<0.05)且显著上调NT蛋白表达(均P<0.01)。干预4 d后,模型组溃疡指数降低但仍高于空白组(P<0.05),PCNA m RNA、SP m RNA、NT蛋白的表达上升且高于空白组(均P<0.01);胃经穴组溃疡指数接近空白组(P>0.05),PCNA m RNA、SP m RNA低于模型组(P<0.01,P<0.05),NT蛋白与模型组比较差异无统计学意义(P>0.05)。干预7 d后,各组以上指标组间比较差异均无统计学意义(均P>0.05)。结论:电针胃经穴能在胃溃疡发展的不同病理状态下对PCNA m RNA、SP m RNA进行双向调节的平衡作用同时促进NT蛋白高表达,进而促进溃疡的修复。
张媛张媛连林宇沈佳成何其达陈娇龙陈娇龙马富强
关键词:电针时效关系增殖细胞核抗原P物质
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