Objective To evaluate effects of monoclonal antibody (McAb) against TNFa on E. coli infected mice. Methods 30 mice (10 week old, Kunming ) were divided equally into three groups. The first group, as a control, 200 μl saline (N S)/mouse was injected intravenously; Second group (untreated group), only E. coli (10 7 organisms/200 μl N S) were injected intraabdominaly; Third group (treated group), E. coli (10 7 organisms) were injected intra-abdominal and McAb against TNFα 2 mg/kg dissolved in 200 μl N S were injected intravenously. After 24 h, observed the survival rate, compared the serum TNF level in blood and investigated pathology of intestine, lung and liver. Results There was a higher survival rate in treated group, with the serum TNF level lowered significantly, and the untreated mice had severe pathologic changes in viscera. Conclusion Using anti-TNFa McAb was effective in reducing mortality rate in mice after infected with E. coli, but could not prevent the pathologic changes.
