The characteristics and configuration of hepatocyte growth-promoting factor freeze-dried powder injection were studied with optical micrography and X-ray diffraction analysis,and the suitable freeze-drying time of hepatocyte growth-promoting factor for injection was determined by the freeze-drying test.The results indicated that the bulk drug and dextran had amorphous configurations and mannitol was crystalline and the freeze-dried powder included tiny mannitol crystals dispersed in the powder uniformly.The crystal form of mannitol in the freeze-dried powder injection changed and the grains became tinier.When the time for freeze-drying was less than 30 h,the powder configuration was not homogeneous.The amorphous dextran was used as the protective agent and disperser for hepatocyte growth-promoting factor,the grains of mannitol constituted the framework of freeze-dried powder injection.It was necessary to take 36 h to produce a good form of hepatocyte growth-promoting factor freeze-dried powder injection.
