[Objective] The aim was to study the genetic diversity of dominant middle-late mature maize hybrids in Jilin Province by the use of Simple sequence repeats （SSRs） molecular mark.[Method] PCR amplification was carried out based on ten core SSR primers and the amplification products were detected through sodium dodecyl sulfate polyacrylamide gel electrophoresis.[Result] Ten core SSR primers gave clear and stable profiles amplified in samples of 43 maize hybrids.A total of 110 polymorphic amplified fragments were produced by 10 primers.The average number of allele per SSR locus was 11 which varied from 4 to 17.The polymorphism information content （PIC） for the SSR loci ranged from 0.63 to 0.93 with an average of 0.842.Genetic similarities among the 43 maize hybrids varied from 0.664 to 0.918 with an average of 0.774 and those among 30 maize hybrids were greater than 0.85.Combined with the cluster analysis and pedigree origin of 43 maize hybrids,it was found that there were more genetic similarities among the 43 maize hybrids.[Conclusion] The germplasm resources of middle-late mature maize hybrids in Jilin Province were comparatively narrow and waited for innovation.
