[Objective] The aim was to research the appropriate seeding quantities of double-season machine-transplanted rice in middle and low-yielding district of southern Jiangxi Province. [Method] The research set five different seeding quantities treatments, and compared with conventional seedling treatment, and the appropriate seeding quantities of double season machine-transplanted rice in middle and lowyielding district of southern Jiangxi Province was discussed. [Result] The yields were highest when the seeding quantity was 70 g per tray of early rice by mechanical transplanting and 60 g per tray of late rice. The yield of early rice by mechanical transplanting showed significantly positive correlation with the number of productive ear and the number of seed per ear, and late rice yield showed significant correlation with the number of productive ear by mechanical transplanting. It is key for improving high yields by guaranteeing the number of productive ear. [Conclusion] The issue of proper sowing quantity should be taken into consideration for double-cropping rice in the region, which is crucial for high yields.