[Objective] The aim was to investigate the effect of four extraction meth- ods on extraction ratio of 11-α-Hydroxy-Canerenone from biotransformation broth. [Method] Previous reported method, soaking, elution method and homogenate meth- ods were used. [Result] Results showed that more than 90% of the biotransformed 11-α-Hydroxy-Canerenone existed in the broth supernatant and mycelia surface. Elu- tion and soaking methods were effective for 11-α-Hydroxy-Canerenone extraction. Extraction yields of 96.0% and 98.8% were obtained by eluting with 400 ml of ethy- lacetate and soaking with ethylacetate for 90 min, respectively. [Conelusion] The method is simple and efficient, showing industrial application potential.
