[Objective] This study aimed to survey the effect of insecticide use intensity on the resistance of rice stem borer in South China. [Method] The data includes 19 counties from Guangdong Province and Guangxi Province from 1991 to 2010. A fixed effect model was established to evaluate the effect of insecticide use intensity on the resistance of rice stem borer. [Result] The regression results suggest that one-period lagged total number of insecticide sprays has significant effect on the infestation level of rice stem borer. [Conclusion] Although rice stem borer is a migratory rice pest, insecticide use intensity has significant effect on the resistance level of rice stem borer in South China.