This study was conducted to investigate the genetic regularity of indexes related to freshness keeping and its molecular basis by acquiring 6 generations (P1, P2, F1, B1, B2 and F2) of an inbred line T3 with long freshness period × an inbred line T15 with short freshness period in sweet corn. The genetic analysis of the indexes was performed by major gene+polygene mixed genetic model combined with the genetic analysis combining six generations.The results showed that the decreasing rate of the postharvest sugar content in the T3 was controlled by two pairs of additive-dominante-epistatic major genes+additive-dominant polygenes; each segregating generation was affected by its major genes, the heritability of major genes and polygene in the B1 generation was 74.63% and 17.67%, respectively; the heritability of major gene and potygene in the B2 was 91.98% and 0,00%, respectively; and the heritability of major gene and polygene inthe F2 was 82.67%, and 12.93%, respectively.