The soil organic matter content in the greenhouses around Lhasa City was determined by potassium dichromate-sulfuric acid digestion method. The results showed that the soil organic matter content differed among different soil layers of the greenhouses around Lhasa City, and it was relatively low in the deep soil. The soil organic matter content also differed among greenhouses with different cultivation modes. In the same soil layer, the organic matter content in arched greenhouse was lower than that in semi-arched greenhouse. Among all the soil samples, the samples with organic matter content below the critical value (20 g/kg) accounted for 61.33%, and the samples with higher organic matter content (≥30 g/kg) accounted for 10.64%. The soil organic matter content in the greenhouses of different counties of Lhasa City ranked as Dazi County's〉Duilongdeqing County's〉Qushui County's. In overall, the organic matter content in the soil of greenhouses around Lhasa City was medium-low, and the fertility of the greenhouse soil was at the middle level.