To observe the effects of moxibustion on β EP receptor from spleen lymphocytes,HAC oncocytes was used to make tumor carrying animal model. The animals were randomly divided into three group, including normal group,control group and therapeutical group. After finish the tenth treatment of moxibustion, blood plamsa was collected to determinate the transformation rate of lymphocytes from spleen and β EP receptor of spleen lymphocytes. The results showed:after vaccination of oncoytes in mice,the contents of β EP in plamsa was in lower state. While after moxibustion of point Guanyuan, the contents of β EP was increased promply and more higher than that in normal groupl. When the concentration of β EP was at 0.3 nm and 0.6 nm,transformation of lymphocytes induced by ConA with low concentration were both enhanced. According to the observation on density of β EP receptor from spleen cell in mice,there was a determinated in moxibustion group. It suggested that moxibustion can not only enhance the contents of β EP in plamsa, but can play a role of positive regulation in β EP receptor from spleen cells. It can be one of the most important mechanism of immunologic regulation by moxibustion.
目的观察艾灸对结肠炎相关性结肠癌(CAC)大鼠的干预作用,从嘌呤受体P2X7R与Wnt/b-catenin信号通路探讨可能的效应机制。方法将SD大鼠随机分为正常组、CAC组、隔药灸组、隔姜灸组。CAC组、隔药灸组、隔姜灸组均采用腹腔注射AOM联合DSS法制备CAC大鼠模型,隔药灸与隔姜灸组均取天枢(双)、气海穴进行治疗。记录各组大鼠体质量、疾病活动指数(DAI)和成瘤率;通过HE染色观察艾灸对CAC大鼠结肠损伤的干预效应;通过RT-q PCR和Western Blot技术,检测艾灸对CAC大鼠结肠组织C-myc、Wnt1、b-catenin、GSK-3bm RNA和P2X7R蛋白表达的调节作用。结果与正常组相比,CAC组大鼠体质量显著降低、DAI增高、成瘤率明显增加(P<0.05),结肠组织可见腺管共壁背靠背和筛样结构,高级别腺癌形成。CAC组大鼠结肠组织P2X7R蛋白表达显著下调(P<0.05),C-myc、b-catenin、GSK-3b、Wnt1 m RNA的表达均显著上调(P<0.05)。与CAC组相比,隔药灸组和隔姜灸组大鼠体质量增加,DAI降低(P<0.05),结肠组织P2X7R蛋白表达显著上调,C-myc m RNA下调(P<0.05);隔姜灸组成瘤率明显降低(P<0.05),且Wnt1、b-catenin、GSK-3bm RNA表达显著下调(P<0.05)。结论隔药灸、隔姜灸均能调节CAC大鼠结肠组织P2X7R及C-myc的异常表达,且隔姜灸还能下调CAC大鼠结肠组织Wnt1、b-catenin、GSK-3bm RNA的表达。
