目的:探讨祛痰活血方对癫痫大鼠炎性因子MRP8(血小板表达谱和骨髓相关蛋白-8)、IL-1β(白介素-1β)的影响。方法:将80只SD雄性大鼠随机分为正常组、模型组、中药组、西药组,各组根据造模成功后的不同时间分为l2、24、48、72 h 4个时相点。戊四氮点燃诱导癫痫模型,各组给与相应药物或生理盐水灌胃,每天1次。ELISA法检测大鼠脑组织MRP8、IL-1β含量。结果:MRP8的水平变化:与模型组比较,中药组和西药组大鼠脑组织MRP8的含量在各时间点均低于模型组(P<0.05);西药组MRP8含量在48、72 h时间点低于中药组(P<0.05)。IL-1β含量的变化:与模型组比较,中药组和西药组大鼠脑组织IL-1β含量在各时间点均低于模型组(P<0.05);西药组IL-1β含量在24、48、72 h时间点低于中药组(P<0.05)。结论:祛痰活血方可以降低癫痫大鼠脑组织MRP8、IL-1β的含量,从而抑制癫痫的炎症反应,减少癫痫发作。
Objective:To observe and compare the clinical effect on knee osteoarthritis(KOA)between Fu’s subcutaneous needling therapy(FSN)and convention acupuncture.Methods:A total of 80 outpatients with KOA were divided into a FSN group(40 cases)and a conventional acupuncture group(40 cases)according to random number table.Within 1 week of treatment,FSN was used once every two days in the FSN group.The needle tip of Fu’s subcutaneous needle was inserted toward the affected muscle,about 5 mm in depth,at the angle of 15 to 25°.After the needle body lifted slightly,the needle went forward subcutaneously for 25 to 35 mm in depth and was swiped side to side horizontally with even exertion for about 1 min.According to the distribution of affected muscle,the reperfusion approach was repeated for 3 times consecutively.In the conventional acupuncture group conventional acupuncture was given,once a day,for 6 times totally.The scores of Western Ontario and McMaster universities osteoarthritis index(WOMAC)and Lysholm knee scoring scale(Lysholm)were compared between the two groups before and after 1-week treatment.The clinical effect of the two groups was analyzed statistically and evaluated.Results:After 1-week treatment with FSN,in the FSN group,the score of WOMACpain was reduced to be7.7±1.9 from 18.5±3.2,the score of WOMACstiffnessto be 1.5±0.7 from 4.5±1.8,WOMACfunction to be22.7±3.9 from 45.7±2.7 and the score of WOMACoverall to be 31.9±5.3 from 69.7±6.5.The differences were significant in comparison before and after treatment(all P<0.05).In the conventional acupuncture group,after 1-week treatment with conventional acupuncture,the score of WOMACpain was reduced to be11.3±2.8 from 18.2±3.0,the score of WOMACstiffness to be 3.0±1.6 from 5.6±1.7,WOMACfunction to be29.8±5.1 from 44.3±2.9 and the score of WOMACoverall to be 44.1±7.8 from 69.1±7.3.The differences were significant in comparison before and after treatment(all P<0.05).WOMAC score of every item in the FSN group was lower obviously than that in the con
