Enzymatic hydrolysis of penicillin G(Pen G)into phenylacetic acid(PAA)and 6-aminopenicillanic acid(6-APA)suffers not only substrate inhibition and non-competitive inhibition by 6-APA,but also competitive inhibition by PAA.In the industrial process,PAA is generally eliminated by addition of aqueous ammonia to form soluble ammonium salt,which generates industrial waste.Extractive biocatalysis is a potential method to overcome the above obstacles and might be even directly used in enzymatic hydrolysis of penicillin G from fermentation broth.The partitioning behavior of Pen G hydrolysis reactants and the stability of immobilized penicillin acylase in cloud point system,a novel extractive bioreaction system,were determined.It suggests that cloud point system might be a novel extractive bioreaction system for the industrial process of penicillin hydrolysis.
