[Objective]The aim was to study cold-resistant indexes and physiological characters of three different cold-resisitant tomato strains selected under natural conditions. [Method]With 3-4 weeks tomato seedlings as tested materials,through simulating external environment conditions by the cold treatment in lab,the survival ratio and growth status of seedlings were identified; by studying the morphological change of in vitro leaves after cold treatment and determining the relative conductivity,the cold-resistant degree of different tomato strains was identified and the correlation between conductivity and survival rate was analyzed; the variation of soluble sugar and praline content in plant of tomato was determined under the condition of cold stress. [Result]The results showed that leaf morphological changes,size of conductivity and growth status of different tomato strains after cold treatment have significant differences; the variation of soluble sugar contents in plant has significant differences,while the variation of proline content has no significant differences. [Conclusion]There is a certain correlation between conductivity and survival rate under cold treatment conditions,and plant cold tolerance is related to accumulation of soluble sugar content.