The solubilities of eugenol, eugenol acetate and β-caryophyllene, which are the main components in clove oil, in supercritical CO2 were separately determined at the temperature range from 40℃ to 60℃ and pressure from 10 MPa to 30 MPa.The results showed that the solubilities of these components increased with increasing pressure or decreasing temperature.The solubility of β-caryophyllene was higher than those of eugenol and eugenol acetate at the same temperature and pressure.The solubilities of eugenol in SC-CO2 were between 0.0002 and 0.0580 (mole fraction).The solubilities of β-caryophyllene in SC-CO2 were between 0.00034 and 0.07096 (mole fraction).The solubilities of eugenol acetate in SC-CO2 were between 0.00018 and 0.07030 (mole fraction).The solubility data of the main components of clove oil in SC-CO2 were correlated with the Chrastil equation and improved Chrastil equations presented by Adachi and del Valle.The values of average-absolute-relative-deviation(AARD) for eugenol solubility correlation were 4.92%,4.47%,5.19% respectively.The values of AARD for eugenol acetate solubility correlation were 3.69%,2.91%,3.24% respectively. The values of AARD for β-caryophyllene solubility correlation were 4.77%,4.41%,4.21% respectively.
为了满足食品及医药等领域的检测需求,研制了黄芩素纯度标准物质。采用液相色谱–质谱法和红外光谱法对黄芩素纯度标准物质原料定性后,利用高效液相色谱法(HPLC)和定量核磁技术(Quantitative Nuclear Magnetic Resonance Spectroscopy,QNMR)对黄芩素的纯度进行了定值,并用HPLC法对黄芩素纯度标准物质进行了均匀性检验和稳定性考察。对定值结果的不确定度进行了评价,研制的黄芩素纯度标准物质的定值结果和扩展不确定度分别为98.8%,0.8%(k=2)。
