[Objective]The aim was to optimize SSR reaction system applied in the identification of authenticity of maize variety.[Method]The technical parameters of SSR including PCR reaction system,annealing temperature and electrophoresis time were optimized to identify 10 major maize varieties in Liaoning Province.[Result]The optimum PCR reaction system was：14.60 μl sterile ultrapure water,2.00 μl 10 × Buffer（Mg2＋）,1.20 μl dNTPs,0.20 μl Taq enzyme,0.50 μl each of the forward and reverse primers and1.00 μl DNA stock solution.Annealing temperature and electrophoresis time could greatly influence the results of PCR amplification.The optimal annealing temperature and electrophoresis time required for the ideal electrophoresis bands under the same conditions were different when different primers were used.[Conclusion]The system was feasible to be applied in rapid identification of authenticity of hybrid maize varieties.
