Nowadays, commercial competition is becoming fiercer and fiercer, and with the globalization proceeding, when a company tries to expand, promote and develop, it needs to adapt itself to the open business society while not losing its own culture. Since English has been widely used in the world, an effective and suitable English website is essential for a company to use as a kind of advertisement and publicity, where its cultural values are presented.With companies' websites as subjects and Hofstede's cultural dimension theory as the theoretical basis, this study, through text content and data analysis of company profiles,strategies and cultures, tries to find out the tendency of Chinese and American companies on the five cultural value dimensions,which is power distance, uncertainty avoidance, individualism, masculinity and long-term orientation. Accordingly, constructive suggestions are put forward to help appease and avoid misunderstandings, and promote business communication and collaboration.