Through study to experiment of eccentricity situation of air column in overflow port, the paper discusses asymmetry of air flow in cyclone separator. The result shows that influence of operation parameter to eccentricity degree of air column in cyclone separator is not obvious, inlet type of cyclone separator is determinative, eccentricity degree is greater while inlet structure is more asymmetric. In addition, it reveals influence of asymmetry of air flow in cyclone separator to separating efficiency. [Descriptors] Cyclone separator Air column Eccentricity Separating