This study was conducted to investigate the effects of bast fiber seedling film on rice seedling growth. In this study, indica cultivar 'Xiangwanxian 13' and indica hybrid cuitivar 'Y Liangyou 1' were used to investigate the changes of aboveground part growth and root respiration enzyme activities of rice seedlings af- ter the application of bast fiber seedling film. The results showed that, compared with the CK, the accumulation of shoot biomass, chlorophyll content, root bleeding intensity, soluble sugar content, and nitrate nitrogen content were significantly im- proved with the application of bast fiber seedling film. In addition, the activities of malate dehydrogenas (MDH) and alcohol dehydrogenas (ADH) were higher in rice seedling roots raised with bast fiber seedling film, while there was no significant change in lactate dehydrogenase (LDH) after the application of bast fiber seedling film. However, there were no significant differences in these indexes between the two cultivars. All the results indicated that the growth and development of rice seedlings could be improved with the application of bast fiber seedling film.