Objective To observe the therapeutic effect of hand-foot-mouth disease treated with pricking moxibustion and analyze the mechanism of action. Methods Seventy-five cases were randomized into 3 groups. Of which, in the combined therapy group of moxibustion and medicine （22 cases）, the pricking moxibustion and routine western medicine treatment were provided in combination; in the Chinese herbal medicine group （29 cases）, oral administration of Chinese herbal medicine and routine western medicine treatment were provided; in the western medicine group （24 cases）, routine western medicine treatment was provided. After 7 days treatment, the therapeutic effects of 3 groups were judged by comparisons of skin rush, oral mucosa herpes, constipation （or loose stools）, indigestion and anorexia, and sore throat. Results The total effective rate was 95.5% in combined therapy group of moxibustion and medicine, 86.2% in Chinese herbal medicine group, 83.3% in western medicine group. The total effective rate in combined therapy group was superior to that in either Chinese herbal medicine group or western medicine group （both P〈0.05）. Comparing the fading durations of skin rush, oral mucosa herpes, constipation （or loose stools）, indigestion and anorexia between combined therapy group and other two groups, significant differences were presented statistically （both P〈0. 05）. Conclusion In the combined therapy group of moxibustion and medicine, the symptoms of alimentary canal were relieved, the disease courses were shortened and the pains were reduced in hand-foot-mouth disease patients.
目的:筛选治疗新型冠状病毒肺炎(Corona Virus Dsease 2019,COVID-19)处方中核心中药的活性分子及其潜在靶标,进一步分析其作用机制。方法:应用Microsoft Excel筛选2020年2月安徽省省级中医专家组及阜阳市中医医院派驻阜阳市第二人民医院参与治疗COVID-19的专家开具的处方中的核心中药;利用中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology da-tabase and analysis platform,TCMSP)过滤中药活性分子及相应靶标,从人类基因数据库(the human gene database,GeneCards)、人类孟德尔遗传数据库(online mendelian inheritance in man,OMIM)寻找COVID-19的相关基因,选取其与中药靶基因的交集,预测中药治疗COVID-19的潜在靶标;使用Cytoscape软件构建中药调控网络图;利用蛋白互作网络(functional protein association networks,STRING)数据库构建潜在靶标的PPI蛋白互作网络,进一步对其中关键靶标进行基因本体(gene ontology,GO)功能富集及京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)信号通路富集分析。结果:处方共使用中药136味,核心中药茯苓、甘草、陈皮、半夏为二陈汤组成药物;获得中药相关靶标223条,COVID-19相关基因364条,中药-“COVID-19”潜在靶标52条,其中核心靶标分别为胱天蛋白酶3(Caspase-3,CASP3)、白细胞介素6(interleukin-6,IL-6)、白细胞介素10(interleukin-10,IL-10)、重组人白细胞介素1β(recom-binant human IL1βprotein,IL-1β)、促分裂原活化蛋白激酶3(mitogen-activated protein kinase 3,MAPK3)、促分裂原活化蛋白激酶8(mitogen-activated protein kinase 8,MAPK8)、信号传导子和激活子3(signal transducers and activators of transcription 3,STAT3)、促分裂原活化蛋白激酶1(mitogen activated protein kinase 1,MAPK1)等,关键活性分子有黄芩苷、β-谷甾醇、柚皮素、川橙皮素、山奈酚、甘草查尔酮A、槲皮素等。结论:核心中药可能通过作用于白�
