[Objective] This study aimed to investigate the residues of rare earth ele-ments in Guangxi banana from banana-producing area with application of rare earth fertilizers and evaluate the safety of using rare earth fertilizers in banana production. [Method] HNO3+H2O2 mixed acid system with high pressure airtight microwave di-gestion sample pretreatment method and ICP-MS technology were used, to establish a determination method of 16 rare earth elements in banana samples, including Sc45, Y89, La139, Ce140, Pr141, Sm147, Eu153, Gb157, Tb159, Nd144, Dy163, Ho165, Er166, Tm169, Yb172 and Lu175. [Result] Different standard curves present-ed good linearity. Detection limit of the instrument was 0.002-0.01 μg/L; detection limit of the method was 0.1-0.6 μg/kg; recovery rate of standard addition was 94.5%-116%; relative standard deviation was 2.02%-14.21%. [Conclusion] This method has many advantages, such as simple mass spectrogram, high sensitivity and high selectivity, accurate quantification, high precision and accuracy, simple operation, high reproducibility and high recovery rate, which is suitable for the detection of rare earth elements in banana and other fruits, with certain theoretical and applicable val-ue for guiding banana production and high-efficient planting.