The heat output of the plant cells-Chlorella vulgaris has been determined using the LKB-2277 bloactivity monitor. The metabolic thermokinetic equation isdP/t = k0, k0 = 0The experimental results indicate that there is a linear relationship between the meatbolic power and the cell concentration and that the heat output produced by a single cell’s metabolism depends oll the cell concentration and is inhibited by the increase of the cell density. It presents a special kind of space effect.
The interactions of two kinds of water-soluble porphyrins, Cu (NEAE) andH2PPS, with DNA have been investigated by microcajorimetry and UV spectroscopy.The experimental results show that Cu (NEAE) interact with dsDNA in the outside-boundmode, its binding number is 9~10 base pairs and its binding enthalpy △H=-24.4kJ.mol-1.There are large enthalpy changes when Cu (NEAE) react with ssDNA. H2PPS can notreact with both dsDNA and ssDAN, which demonstrats that the coulombic force is one ofthe important factors affecting the interactions of porphyrins with DNA.