[ Objective] The aim of the study is to clone the 5＇ flanking region of Sporamin A gene from sweet potato. [ Method ] The sweet potato ＂Xushu 18＂ was used to amplify the 5＇ flanking region of Sporamin A gene using specifically designed primers and then target fragment was analyzed by PLACE and PlantCare online. [ Result ] Besides the conserved elements including TATA-box and CAAT-box, the cis-acting elements including sucrose responsive element CMSRE1, SP8 acting site and some other regulatory sequence such as MYB binding site were also found in Spo A promoter sequence. The results suggest that the promoter has sucrose responsive function. [ Conclusion ] The study provided reference to reveal the regulation law of Spo A, and to develop high-level expression vectors promoted by Spo A promoter.
