[Objective] The aim of this study was to explore the taxonomic status and genetic relationship of ants at molecular level.[Method]Applying cyt b gene as a molecular marker,molecular phylogenetic analysis of 14 ant species of 5 genera(Camponotus,Formica,Polyrhachis,Pheidole and Crematogaster)in Formicidae was conducted.Partial sequences of cyt b gene in 14 ant species were analyzed with software MEGA,Clustal X and PAUP,and phylogenetic trees were constructed by Neighbor-Joining method(NJ)and Maximum-Parsimony method(MP).[Result]NJ tree and MP tree showed that the 14 ant species could be clustered into 5 branches.[Conclusion]The results of molecular phylogenetic analysis coincided with the views of traditional morphological taxonomy.