The method of polymrase chain reaction(PCR) was used to amplify the insertion sequence-like element 6110 which is specific toMycobacterial tuberculosis complex (MTBC). A123 base pairs fragment was specificallyamplified from the MTBC genome. Neitheratypical Mycobacteria nor other species bacteriawas found this amplification.The PCK candetect the purified MTBC genome in as littleas 10 fg. In the detected urine sample of the59 episodes, the specific PCK positiuity andnegatiuity was 83.33% and 100% respectively.It is indicated that the diagnosis of PCK forrenal tuberculosis is senitive, specific and rapid,and should be developed in future.
