[Objective] The aim was to explore interrelationship between agricultural input and output in Jiangsu and the influence degrees of input factors on agricultur-al output. [Method] Quantitative analysis and evaluation were made on agricultural input and output in Jiangsu during 1990-2012 as per factor analysis and regression analysis. [Result] The result of factor analysis showed that since the 1990s, the comprehensive efficiency of agricultural input/output in Jiangsu was growing and in-put/output of agriculture, forestry, animal husbandry and fishery, crop farming, and of food production were extracted, whose scores reflect the changes of input/output ef-ficiencies in terms of agriculture, forestry, animal husbandry, fishery, crop farming and food production in the two decades. The results of regression analysis indicated that the effects of the three indices on agricultural output tended to be volatile and the influence degrees were concluded also by regression parameters. [Conclusion] The research provides theoretical references for agricultural input/output structure in Jiangsu Province.