[Objective] To study the characteristics and effects of inorganic nitrogen in east water-source and inflow rivers of Chaohu Lake. [Method] The speciation and distribution characteristics of dissolvable inorganic nitrogen (DIN) in east water-source and inflow rivers of Chaohu Lake were investigated, and their effects on water qual- ity were examined. [Result] The concentrations of NH3-N and NO2--N were the high in flood season, and low in non-flood season, while the concentration of NO3--N pre- sented the opposite trend; the concentration of NO3--N was the highest in Shuangqiao estuary, where the pollution was the worst. DIN in Zhegao estuary and Xiaozhegao estuary was mainly caused by domestic sewage and industrial wastewaters; surface runoff and pollution from ships contribute the most to the DIN content in Shuangqiao estuary. [Conclusion] This study provided basic data and theoretical basis for the control and management of eutrophication in Chaohu Lake.