The pure product of 2-aminophenoxazine-3-one was prepared by enzyme-catalyzed reaction and analyzed. The electrochemical behaviour of 2-aminophenoxazine-3-one on a glass carbon electrode was investigated. The interaction between DNA and 2-aminophenoxazine-3-one was investigated by cyclic voltammetry and UV-Vis spectroscopy. The peak potential of 2-aminophenoxazine-3-one in pH 6.8 phosphate buffer solution in the presence of DNA shifts positively, indicating that the bind of 2-aminophenoxazine-3-one with DNA is intercalation action. The influence of reacting time was also studied. The binding ratio of the complex of DNA and 2-aminophenoxazine-3-one at room temperature is calculated to be 1∶1, and the binding constant is to be 6.60×10 4 L/mol.
