[Objective] This study was conducted to compare total contents of poliumoside and forsythoside B from Callicarpa kwangtungensis Chun in Qiandongnan Miao and Dong Autonomous Prefecture collected in different seasons, which could provide reference for its deep development and utilization. [Methods] Poliumoside and forsythoside B were measured according to the pharmacopoeia standard in the middle of each month in 2014, and the yield of C. kwangtungensis was simultaneously evaluated. All these results provided data reference for the determination of suitable picking time for C. kwangtungensis. [Results] The results showed the content of poliumoside and forsythoside B in C. kwangtungensis was the highest in November, and the content of the medicinal material in August was over eight times higher than the pharmacopoeia standard, besides at this month the yield was the highest during the year. Comprehensively, mid October is the optimum picking time for C. kwangtungensis in Taijiang. [Conclusion] Dynamic variations of content of poliumoside and forsythoside B in and yield of C. kwangtungensis were investigated, which would significantly benefit production of C. kwangtungensis in Qiandongnan Miao and Dong Autonomous Prefecture.