为了探讨DNA条形码技术在夜蛾物种鉴定中的可行性,本研究利用条形码通用引物扩增了北京百花山地区43种夜蛾75个样本的线粒体细胞色素C氧化酶亚基I(mitochondrial cytochrome c oxidase subunitI,COI)基因序列,以Kimura双参数模型进行种内种间遗传距离分析、使用邻接法(neighbor-joining,NJ)和最大简约法(maximumparsimony,MP)分别构建系统发育树,并利用分子序列差异阈值对样本进行分子可操作分类单元(molecular defined operational taxonomic units,MOTU)划分。结果表明:所有夜蛾种类通过系统发育树可以成功区分;种内平均遗传距离(0.03%)远远小于种间平均遗传距离(11.29%);采用较为保守的1%的序列差异阈值将75个夜蛾样本分为42个MOTU,正确率为95%,除了MOTU04包含2个物种外,剩余41个MOTU与形态种呈现一一对应的关系。结果显示,基于COI基因的DNA条形码对于本研究中所涉及的夜蛾具有较好的区分,可以作为一种有效的工具在夜蛾科昆虫物种鉴定中进行应用。
This paper reports two noctuid moths, Atrachea japonica （Leech, 1889） and Autographa macrogamma （Eversmann, 1842） that are new to the Chinese fauna. They are distributed in Tahe County in Daxing＇anling forest region, China. In addition, a new recourd species, Neustrotia rectilineata Ueda, 1987, which is described from Mt, Sudo-san, Korea and Hotso, Prov. Taiwan, is newly added to continental fauna of the family Noctuidae s.l.. The external characteristics, genitalia photos, and the distributional ranges of those species are provided, and all the materials examined in this study are deposited in the collection of the Entomological Laboratory, Forestry college of Northeast Forestry University, Harbin, China.
