Cimicifugae Rhizome （RC） is one of many commonly used traditional Chinese medicines. This study used Box-Behnken Design （BBD）, a widely used form of response surface methodology （RSM）, to optimize the conditions of the ultrasound-assisted extraction （UAE） for the extraction of phenolic compounds from CimicifugaeRhizome. The influence of three independent variables, including ethanol concentration （%）, extraction time （min） and solvent-to-material ratio （mL/g）, on the extraction yields of phenolic compounds were examined. The results demonstrated that the optimal ultrasound-assisted extraction （UAE） conditions were as follows： ethanol concentration of 68%, an extraction time of 19 min, and solvent-to-material ratio of 30 mL/g for the extraction of caffeic acid, cimicifugoside, ferulic acid, isoferulicacid, cimifugin, 4′-O-β-glucopyranosyl-5-O-methylvisamminol. These results demonstrated the suitability of UAE for the extraction of phenolic compounds from CimicifugaeRhizome.
目的：建立同时测定保健茶中9种有机氯农药残留量的毛细管气相色谱法。方法采用DB‐1701弹性石英毛细管色谱柱（30 m x0．25 mm ，0．25μm）分离，63 Ni电子捕获检测器测定。结果9种有机氯农药质量浓度在1～100 ng· mL －1范围内呈良好线性关系；平均加样回收率为86．34％～119．80％，RSD为7．36％～8．37％（n＝9）。结论该方法简便快速准确，可用于测定保健茶中9种有机氯农药的残留量。
