Effect of soil acidification on yield of late rice was studied and acid resistance of late rice varieties were compared with 23 late rice varieties as materials in Changsha County, Hunan Province. The results indicated that the difference in yield among varieties was obvious, yield in common field was among 5 226.6-9 202.1kg/hm^2, and yield in acidified field was among 3 643.2-7 714. 8 kg/hm^2. Compared with common field, yield of Yueyou 6135, Huayou 18, Jinyou 284 and Ⅱyou 46 increased by 3.24%-26.33% in acidified field, while yield of other varieties decreased by 2.04%-56.79% in acidified field. According to acidification sensitivity, Wufengyou T025, Jinchuyou No.148, Yueyou No.6135, Shenyou No.9586, Xiangfengyou No.103,Zhongyou No.288, Nongxiang No.18, Shanyou No.432, Ⅱ you No.6, and Zhong 9A/R10402 were sensitive to soil acidification; Wuyou No.308, Zhunliangyou No.608,Fengyuanyou No.227, Fengyou No.1167, Fengyuanyou No.299, T you No.272, and Zhong 9A/R9963 were moderately sensitive to soil acidification; Yueyou No.9113,Jinyou No.284, Shenyou No.9588, Huayou No.18, Ⅱ you No.46 and Ⅱ you No.3027 were slightly sensitive to soil acidification