[Objective] This study aimed to determine the optimum ethyl methane sulfonate（EMS） concentration for germination of tarary buckwheat seeds so as to lay a solid foundation for obtaining excellent traits of tarary buckwheat and breeding new varieties that meet the requirements by EMS mutation breeding. [Method] The seeds of Jinqiaomai No.4, a local tarary buckwheat variety [Fagopyrum tataricum（L.） Gaertn] in Shanxi Province, were used as the material. They were mutagenized by different concentrations（0.3%, 0.5%, 0.7%, 1.0%, 1.5%, 1.7%） of EMS for different times（4, 8, 12 h）. Then the germination rate and germination vigor were analyzed. [Result] With the increase of EMS mutagenic agent concentration, the germination vigor, relative germination vigor, germination rate and relative germination rate are all trended to be decreased. When the EMS concentrations ranged from 0.3%to 1.0%, there were no significant differences between treatment and control groups（P〉0.05）. When the EMS concentrations were 1.5% and 1.7%, significant differences were shown between treatment and control groups（P〈0.01）. The 4 and 8 h treatment of EMS all had no significant effects on germination rate and relative germination rate of Jinqiaomai No.4, but the 12 h treatment of EMS showed significant effects. Among the three treatment times, there were still no significant differences in germination vigor and relative germination vigor between treatment and control groups. [Conclusion] The optimum median lethal dose and treatment time of EMS for mutagenizing Jinqiaomai No.4 were 1.7% and 12 h, respectively.
以AnHRGP基因编码的富含羟基脯氨酸糖蛋白(AnHRGP)为研究对象,用生物信息学方法对该蛋白质的一级结构及理化性质、跨膜区、亲疏水性、二级结构及功能位点、三级结构特点等进行分析。应用ExPASy在线分析AnHRGP的氨基酸组成及理化性质,通过SOMPA在线预测AnHRGP的二级结构,应用TMHMM Server v. 2.0对AnHRGP进行跨膜结构分析,采用Phyre^2对蛋白质的三级结构进行建模。结果表明,该蛋白基因的开放阅读框(openreadingframe,简称ORF)可以编码202个氨基酸,是一种无信号肽、无跨膜结构、无卷曲螺旋结构、定位于叶绿体和线粒体基质空间的不溶性疏水蛋白。无规则卷曲为AnHRGP中成分最多的二级结构。通过Phyre^2工具模拟出了该蛋白质的三级结构模型。
