[Objective] This study aimed to test whether salicylic acid (SA) can im-prove the physiological functions of flue-cured tobacco under subdued light condition, and to determine the mechanism of its action. [Method] The tobacco plants under subdued light were foliar-sprayed with 100 mg/L of SA. Then, the physiological in-dices such as plant fresh weight and dry weight, chlorophyl content, photosynthetic parameters and chlorophyl fluorescence parameters were measured. SPSS17.0 and Excellwere adopted for variance analysis and significance test. [Result] The leaf photosynthetic rate (Pn), stomatal conductance (Gs) and transpiration rate (Tr) of tobacco plants in subdued light were al decreased while the intercellular CO2 con-centration (Ci) was increased, suggesting that non-stomatal limitation led to the de-crease of Pn under weak light intensity stress. SA released the inhibition of tobacco plant growth in weak light, as it elevated the leaf photosynthetic rate, the maximum photochemical efficiency of PSⅡ, potential activity of PSⅡ, effective photochemical efficiency of PSⅡ and photochemical quenching coefficient in weak light significant-ly, and reduced the non-photochemical quenching coefficient. [Conclusion] SA has significant effects on the photosynthetic characteristics of flue-cured tobacco in weak light, and it can improve the synthesis or distribution of photosynthesis product, and the efficiency of light energy, conducive to plant growth and development.