SDS-PAGE were employed to detect the inhibitions of some antioxidants to the degradation of bovine serum albumin induced by AAPH.The result showed that Rosmarinic acid,uric acid,sodium deoxycholate and GSH exhibited inhibitions at low concentration(125μmol/L),but thiourea needs high concentration(250μmol/L).Nicotinicacid and Vc did not show obvious protection even the consistence up to 2000μmol/L.Mannitol’s behavior was neither clear no concentration-dependent.Thymol blue bromide,pyrogallic acid and Trolox are the strongest inhibitors.They manifested obvious protections at 62.5μmol/L.
The ability of several nature antioxdants to protect free radical initiator AAPH induced oxidative DNA damage was assessed by measuring the conversion supercoiled pBR322 plasma DNA to the open circular and linear forms.It was found that six nature antioxidants could significantly inhibit the oxidative DNA damage induced by AAPH and their abilities ranked one by one in order as rutin,quercetin and vanillin,ursolic acid,curcumim,oleanolic acid.
