内质网应激(ERS)与病毒性肝炎的发生及转归有极大关联。而肝细胞未折叠蛋白反应是介导ERS的重要环节,持续强烈的ERS会激活肝细胞的凋亡信号通路。其中,78 k Da葡萄糖调节蛋白(GRP78)、葡萄糖调节蛋白94(GRP9)是未折叠蛋白反应的标志性蛋白,生长抑制DNA损伤基因153(CHOP)是细胞凋亡通路的标志性蛋白,且GRP78、GRP94和CHOP与病毒性肝炎有密切联系,但具体机制尚未阐明。随着对未折叠蛋白反应和凋亡通路影响因子研究的不断深入,未来对ERS与病毒性肝炎发病机制的研究重点在于明确ERS是如何被病毒性肝炎诱导产生的及ERS如何调控病毒性肝炎的复制。
The Mycobacterium tuberculosis NDA in the macrophages from the different clinical specimens,such as ascites,pleurorrhea,CSF,BALF and blood,is detected with polymerase chain reaction (PCR).The results indicate that the test specificity is 96%,the precision is 92% and the sensitivity is 88%.The Separated and purified macrophage in a good specimen in the detecting TB-DNA by PCR.The test specificity and precision are high.The test is a good method of the diagnosis of the tuberculosis,specially the patient with the outer-pulmonary tuberculosis.The exact significance in the basic theory and the clinical practice are not affirmed.so it will be investigated further.
