传统的形态鉴定主要依赖于分类者的经验和水平,容易产生分类错误。为提高物种鉴定的准确性,作者采用形态学与线粒体16S rRNA基因序列分析相结合的方式对广西北海涠洲岛采获的3种珍珠贝进行了鉴定。研究结果表明,基于形态学可将3种珍珠贝分别鉴定为企鹅珍珠贝(Pteria penguin)、宽珍珠贝(P.loveni)和中国珍珠贝(P.chinensis),但基于16S r RNA基因序列,形态学鉴定为P.loveni的标本应为P.lata。P.loveni和P.lata是两个完全独立的种,并非为同物异名。珍珠贝属16S rRNA基因序列的种间遗传距离明显大于种内遗传距离,适宜作为该属种类鉴定的分子标记。在基于16S r RNA基因序列构建的系统树中,相同种类的不同个体均形成单系群,并获得很高的支持率。分布于中国沿海的珍珠贝属种类应包括P.lata,是否有P.loveni的分布尚需进一步证实。
A 3-D unstructured-grid, finite-volume coastal ocean model (FVCOM) was used to study the tides and tidal currents in the Lianzhou Bay and adjacent areas. The simulation results were in good agreement with observations. The co-tidal maps of K1 , O1 , and M2 indicated that K1 and O1 were mostly standing waves outside the Lianzhou Bay, while M2 had a largely propagating character. However, all three constituents became propagating waves when entering the Lianzhou Bay, due to the shallow waters. The tidal current ellipses showed the characters of K1 , O1 , and M2 constituents: K1 and O1 were rotating outside the bay, but rectilinear along the water channels inside the bay; M2 was mostly rectilinear over the whole area. The tidal-induced residual current shows the flow was divided into two branches by the Guantouling Peninsula: one turned to flow west; the other was blocked by the southern boundary of the peninsula, creating a clockwise circulation. In Lianzhou Bay, there were two circulation systems, a cyclonic one at the top of the bay and an anti-cyclonic at the mouth.