An analytical method based on reversed-phase liquid chromatography has been developed for the simultaneous determination of 21 sulfonamides in cosmetics.Various cosmetic samples,including creams,lotions,powders,shampoos and lipsticks,were extracted under ultrasonication,and then separated on a Waters Symmetry C18 column(5 μm,4.6 mm×250 mm) by gradient elution with methanol and 0.1% formic acid in water as the mobile phase.In the range of 1-800 ng,the calibration curves of 21 hormones showed good linearity with the correlation coefficients more than 0.999.The positive results were identified and verified by HPLC-MS/MS.The mean recoveries at the three spiked levels of various cosmetic samples were 89%-109% with the relative standard deviations of 0.7%-6.6%.
