A number of microorganisms in activated sludge in the process of waste water treatment were used for accumulating polyhydroxyalkanoates(PHA). Mixed bacteria and organic carbon source were used in microaerophilic-aerobic treatment of activated sludge and some cultural conditions: oxygen supply rate, attachment carbon source concentration and culture time were investigated. The PHAs obtained appeared a mixture of copolymers or heteropolymers with various monomer unites:3-hydroxybutyrate, 4-hydroxy- pentanoate, 3-hydroxydodecanoate, 3-hydroxy-tetradecanoate, 3-hydroxy-9-hexadecanoate, 3-hydroxyhe- xadecanoate, 3-hydroxy-11-octadecanoate, 3-hydroxy-9-octadecanoate, and 3-hydroxyocta- decanoate.
It is found that a number of microorganisms in activated sludge in the process of waste water treatment can produce a variety of nomo and co poly 3 hydroxyalkanoates with a wide range of properties, which would be one of the methods to low the cost of PHA. In this paper, the biosynthesis of polyhydroxyalkanoates(PHA) by mixed bacterium growth on waste water was studied. The chemical structure of PHA, isolated from activated sludge in waste water treatment process using sodium acetate as attachment carbon source, was analyzed by 1H NMR spectroscopy and gas chromatography mass spectrometry. The PHA produced from activated sludge is a copolymer composed of 9 different monomer units, 3 hydroxybutyrate, 4 hydroxypentanoate, 3 hydroxydodecanoate, 3 hydroxy tetradecanoate, 3 hydroxy 9 hexadecenoate, 3 hydroxyhexadecanoate, 3 hydroxy 11 octadecenoate, 3 hydroxy 9 octade\|cenoate, 3 hydroxyoctadecanoate. [WT5HZ]
