A kind of mixed bacterial manure was prepared,which was composed of four bacterium,namely,Bacillus megatherium var.phosphaticum,B.mucilaginosus,Azorhizobium sp.and cellulose-decomposing microorganisms.Preservation experiments of the mixed bacterial manure were carried out under different temperature conditions.The results showed that when the temperature was 4,18 and 28 ℃,the colony counts of each preserved bacterium increased until 30 days and then almost all of them began to decline,and the total colony counts of the bacterial manure varied little among 30 days and its growth curve reached a peak and a valley when the temperature was 4 and 38 ℃ respectively.This results suggested that the bacterial manure was optimum to be preserved at 4 ℃ and be kept for 30 days,and the conditions 4—28 ℃ and 10—90 days were the matched ranges for its preservation.Potting experiments showed that at room temperature and the amount of inoculation was ≤5‰,the growth curves of Azorhizobium sp.and cellulose-decomposing microorganisms were very similar and both hadn’t a great change,but that of B.megatherium var.phosphaticum and B.mucilaginosus varied significantly with the increasing amount of inoculation. This suggested that at room temperature,the total colony counts of the bacterial manure would increase when the amount of inoculation was ≤5‰ and reached the highest value 90 days later after soil inoculation.
