目的建立同时测定大黄药材中的芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚的方法。方法采用反相高效液相色谱（RP-HPLC）法对大黄药材中5种蒽醌及其苷的含量进行测定。色谱条件如下，色谱柱：E Merek Li Chrospher 100RP-18（4．6mm×250mm，5μm），流动相为甲醇-0．5％高氯酸（85：15），流速0．8ml／min，柱温40℃，检测波长为254nm。结果在此色谱条件下，各蒽醌成分达到基线分离，且峰形良好。芦荟大黄索线性回归方程为：Y=-2410＋5374661X，r=0．9994，在0．016～0．160μg范围内呈良好线性关系，其平均回收率为104．8％，RSD=1．65％；大黄酸线性回归方程为Y=2148＋5440831x，r=0．9997，在0．012—0．120μg范围内呈良好线性关系，其平均回收率为104．0％，RSD=2．71％；大黄素线性回归方程为Y=1653＋4923143X，r=0．9998，在0．017—0．166lag范围内呈良好线性关系，其平均回收率为101．4％，RSD=1．86％；大黄酚线性回归方程为Y=5823＋6496978X，r=0．9998．在0．029—0．286lag范围内呈良好线性关系．其平均回收率为103．2％，RSD=1．93％；大黄素甲醚线性回归方程为Y=3827＋3385073X．r=0．9996，在0．008—0．079Ixg范围内呈良好线性关系，其平均回收率为102．0％，RSD=1．85％。结论分析方法快速、灵敏、准确，所有待测组分峰分离度良好，达到定量检测的要求。
The major metabolites of ginsenoside-Rb1 in rats urine were described.Urine samples were collected before and after 24 h of single oral administration of 150 mg and intravenous administration of 60 mg Rb1 to four rats,respectively.The samples were purified by SPE column and then were analyzed by liquid chromatography-ESI-mass spectrometry for potential metabolites.Parent drug and its fourteen metabolites were identified in rat urine based on the comparison of total ion chromatograms of the blank with the metabolic urine as well as mass spectra.Its main metabolic pathways and possible structures are analyzed in detail.
