Protein in alcohol diversity was tested for the endosperm of Pinus massoniana from natural population using vertical slab polyacrylamide gel electrophoresis. 26 patterns of protein in alcohol diversity and 11 allele loci were obtained. The results indicated the ratio of polymorphic loci was 72.7%( P ), mean number of alleles per locus was 3.636( A ), mean effective number of alleles per locus was 2.663( Ne) ,mean expected heterozygosity per locus was 0.552( He ), mean observed heterozy gosity per locus was 0.231 (Ho) and fixation index was 0.557>0( F) .It showed the population had higher genetic diversity, and pure individual was excessive.
