进行了甘蔗属近缘野生种——斑茅抗逆性基因克隆的研究。以斑茅为材料,用RACE技术克隆了斑茅的20S蛋白酶体α亚基5基因,其编码区为711 bp,编码237个氨基酸,并于genbank分析其核苷酸和推导的氨基酸序列的同源性,用Clustalx version 1.8软件分析其系统进化关系。结果表明,物种间蛋白酶体进化保守,其核苷酸序列与水稻、大豆、剑叶藓属植物的相应基因同源性分别为91％、81％、80％;其氨基酸序列与水稻、大豆、剑叶藓属植物的同源性分别达97％、95％、86％,与人的同源性也高达69％。
Betaine was accumulated as a nontoxic and protective osmolyte in water-dificit and salt-stressed plants.Be-taine aldehyde dehydrogenase for glycine betaine synthesis is an important enzyme.The BADH gene of Maizewas cloned by RT-PCR and RACE.The gene is 1 762 bp in length,including an open reading frame of 1 515 bp.Its nucleotide sequence shares 95% with the the partial cDNA of BADH1 from Sorghum bicolor.Its deducedamino acid sequence contains the conserved domain sequence 'VTLELGGKSP' of ALDH,and a tripeptide SKLat its C-terminal,a signal targetting to the microbodies.The phylogenetic tree of 20 BADHs was constructed,which corresponds to the classical botanical division of plant,the BADH of maize is closest to the one of Oryza Sativa.GenBank accession No.AY587278.
