[Objective] The aim was to study the conditions of tissue culture regeneration seedling by using the stem segments of Euphorbia tirucalli and determine the optimum culture condition of each culture stage,so as to provide references for the factory production and relative study of tissue culture seedling of E. tirucalli. [Method] Taking the stem segments of E. tirucalli as explants,the effects of various mediums on germination rate,multiplication coefficient and rooting rate were studied. [Result] The optimum induction medium of germination culture was 1/2MS＋NAA 0.02 mg/L＋6-BA 1.0 mg/L,with differentiation rate of 89.7%; the best subculture medium was 1/2MS＋NAA 0.02 mg/L＋6-BA 0.60 mg/L＋AD 3.0 mg/L,with multiplication coefficient of 5.70; the optimum rooting culture medium was 1/2MS＋NAA 0.40 mg/L＋IBA 0.4 mg/L,with rooting rate of 100% and transplanting survival rate of 80%. [Conclusion] The tissue culture conditions of stem segments of E. tirucalli were determined primarily.
以香蕉吸芽为材料,研究不同季节取材、消毒方法、继代培养容器外表面的臭氧灭菌处理和简易大棚炼苗环境的甲醛熏蒸对控制香蕉工厂化繁殖中微生物污染的效果。结果表明,采用0.2%HgC l2浸泡10 m in后,换0.2%HgC l2再浸泡10 m in的消毒方法获得无菌外植体的成功率较高,且外植体低代增殖率较高;秋冬季采芽获得无菌外植体的成功率显著高于春夏季;臭氧处理继代培养容器外表面既可以显著降低继代丛芽的污染率,又能够显著地提高工作效率和节约劳动成本;甲醛熏蒸简易大棚内环境可以显著降低香蕉试管苗炼苗期间的微生物污染,提高出苗率。
